Bio-Rad’s new chemiluminescent detection reagent, Clarity ECL, meets the diverse needs of researchers who use Western blotting.
The Hercules, California, US-based company says the reagent provides excellent results for high- and low-expression proteins, using either film or digital detection systems.
‘The Clarity western ECL substrate takes the guesswork out of substrate selection and eliminates the risk of choosing incorrectly,’ says Ryan Short, western blotting product manager at Bio-Rad. ‘By combining superior performance and an affordable price, the Clarity substrate offers researchers nearly universal application.’
The versatile reagent is for blots using nitrocellulose, PVFD, and low-fluorescence PVDF and can be used to detect both high- and low-abundance proteins when using film or CCD imaging systems for blot detection. The material has a one-year shelf life and is stored at room temperature.
Bio-Rad says Clarity ECL offers a number of advantages with respect to sensitivity, background, and signal duration:
- Sensitivity — detects proteins at mid-to-low femtogram levels
- Low background — generates clean-looking blots with bright bands of interest and low background luminescence
- Long signal duration — provides signal duration of 24 hours, giving researchers the flexibility to take longer exposure times or multiple exposures before the signal starts to fade.
