Next-generation large molecule affinity purification solution improves bioanalysis

Thermo Scientific says the MSIA Streptavidin EVO microcolumns simplify large molecule bioanalysis

Thermo Scientific MSIA (Mass Spectrometric Immunoassay) Strepdavidin EVO microcolumn pipette technology used with Tecan EVO Freedom robotic platform for an automated affinity purification solution. (Photo: Business Wire)

A new, fully automated affinity purification solution enables scientists to analyse large biomolecules, such as proteins and antibodies, with improved accuracy, consistency and simplicity, even at high throughputs.

The new Thermo Scientific MSIA Strepdavidin EVO microcolumns bring the mass spectrometric immunoassay (MSIA) affinity purification technology to Tecan’s Freedom EVO series of robotic platforms that are equipped with a 96 multichannel arm.

Biopharmaceutical, translational, clinical and sports anti-doping labs performing high-throughput characterisation of large molecules are commonly challenged by the limitations of conventional affinity purification methods. These challenges include low efficiency, inconsistent results, high background and carryover and the inability to standardise methods and transfer between labs.

When combined with the Tecan Freedom EVO robotic platform, MSIA Streptavidin EVO microcolumns enable fast and accurate isolation of target analytes from complex biological matrices, Thermo Fisher says.

Housed within a pipette tip, the proprietary monolithic columns are densely coated with streptavidin for analytical affinity purification of any biotinylated affinity ligand, even at low concentration. Since this technology is incorporated into a pipette tip, users can analyse sample volumes as low as 10µL, saving precious samples.

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