Bio-Rad's ProteOn and Integral Molecular's Lipoparticles provide high throughput kinetic analysis of protein interactions
Bio-Rad Laboratories, a US-based manufacturer of life science research and clinical diagnostic products, is to work with Integral Molecular to offer a system for characterising the binding affinities of antibodies to diverse integral membrane proteins.
Bio-Rad Laboratories, a US-based manufacturer of life science research and clinical diagnostic products, is to work with Integral Molecular to offer a system for characterising the binding affinities of antibodies to diverse integral membrane proteins.
Integral Molecular, a solution provider for applications involving integral membrane proteins, has developed Lipoparticle, a technology that enables integral membrane protein interactions to be measured using Bio-Rad's ProteOn XPR36 protein interaction array system.
According to Bio-Rad, understanding antibody binding characteristics is critical for screening new drug candidates and developing reagents. Current methods for measuring the binding affinity of ligands to transmembrane proteins require whole cells or cell membrane preparations, which are heterogeneous and too impure to generate high quality, reproducible biosensor data.
Integral Molecular's technology offers a standardised reagent with higher concentrations of membrane proteins than cell membrane preparations. Lipoparticles exploit the self-assembling machinery of non-infectious viral core proteins to create non-living, stable, homogenous nanoparticles comprising a lipid bilayer incorporating highly concentrated target receptors. Because the lipid bilayer is derived directly from mammalian cells, the incorporated membrane proteins are structurally intact and correctly oriented.
Bio-Rad's ProteOn XPR36 is a multiplexed 6x6 SPR array that permits the label-free kinetic analysis of 36 different protein interactions in a single experiment.
"˜Combining Bio-Rad's ProteOn system with Integral Molecular's Lipoparticles results in a high throughput workflow for screening highly concentrated, native membrane protein targets and their binding partners in hours instead of days,' said Renee LeMaire-Adkins, protein function division marketing manager at Bio-Rad.
