Cell culture media provide the proper composition of nutrients for healthy cell propagation and high protein expression. Discover how single-use mixers can simplify dissolution and increase product safety
Cell culture media are complex mixtures of synthetic and biological components that provide the proper composition of nutrients for healthy cell propagation and high protein expression. Key concerns are to ensure product safety and purity. Dissolution of cell culture media can be facilitated by single-use mixing systems. Disposable mixers and filtration assemblies provide operational advantages by eliminating cleanin-place (CIP) and steam-in-place (SIP) process steps, reducing water usage and water for injection (WFI) consumption.
In addition, the use of disposables can reduce the risk of microbial contamination. This case study presents our recommended methods for thoroughly mixing media, as well as reducing the risk of mycoplasma contamination through the use of 0.1 μm sterilizing-grade filters.