HPTLC – a thoroughly modern method

Published: 28-May-2010

High Performance Thin Layer Chromatography (HPTLC) is firmly established as a fast and effective analytical tool, yet in many laboratories traditional TLC is still the norm.

High Performance Thin Layer Chromatography (HPTLC) is firmly established as a fast and effective analytical tool, yet in many laboratories traditional TLC is still the norm.

Omicron Research Limited, CAMAG’s UK distributor, brings you the 104th edition of CAMAG’s Bibliography Service (CBS), featuring HPTLC -aided research from all over Europe, plus more than 200 extracts from TLC related studies.

A new HPTLC method has made it possible for the first time to validate the claims of aloe vera concentrations in retail products, while another extremely valuable application of HPTLC is in the investigation of triterpenoid in plant extracts. This provides unique separations with a wealth of potential mobile phases, with possibilities for in situ derivatisation, resulting in characteristic specific coloration and fluorescence of the separated bands.

The Saint Petersburg Institute of Pharmacy currently uses more than 20 validated, quantitative HPTLC methods, proving particularly helpful for optimisation of extraction and determination of antioxidative activity. Other important uses are the quantification of chlorogenic, caffeic and ferulic acids in coffee bean extracts, curcuminoids in plasma and dosage forms, bile acids and cholesterol in the bile.

Copies of CBS 104 are available from Omicron on info@omicron-uk.com or +44 1488 682700.

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