Lonza launches new medium to optimise the production of AAV production in Sf9 insect cells

To date, translational scientists and researchers have been challenged with the lack of a medium dedicated to AAV production in Sf9 insect cells. The new high-performance medium accelerates cell growth, increases productivity, and reduces process variability and costs, expediting time-to-market for safe, scalable, life-saving gene therapies.

Lonza announces the launch of the TheraPEAK SfAAV Medium, the first chemically defined, non-animal origin medium designed specifically for the production of Adeno Associated Virus (AAV) in Spodoptera Fuigiperda (Sf9) insect cells for gene therapy applications. The new high-performance medium accelerates cell growth, increases productivity, and reduces process variability and costs, expediting time-to-market for safe, scalable, life-saving gene therapies. The unique benefits of the TheraPEAK SfAAV Medium, which marks the latest addition to Lonza’s TheraPEAK Family of cell culture media and reagents for cell and gene therapy applications, are summarized in an informative poster that can be downloaded for free from the Lonza website.

Gene therapy holds great promise for the treatment of rare disorders and diseases, and viral vectors are commonly used to facilitate the delivery of the gene of interest into patient cells. AAV has been established as a viral vector of choice, due to not replicating in patients, thus posing a lower health risk. Owing to their ability to be grown at high densities, the Sf9 insect cells are ideal for use as hosts for the production of large AAV quantities. To date, however, translational scientists and researchers have been challenged with the lack of a medium dedicated to AAV production in Sf9 insect cells.

Lonza’s TheraPEAK SfAAV Medium has been explicitly designed to address this need, providing a cost and time efficient solution for the production of AAV in Sf9 insect cells. Allowing for rapid cell growth, the TheraPEAK SfAAV Medium reduces processing time significantly, enabling cell infection one day earlier than similar media available on the market, and boosting laboratory performance. Due to its chemically defined nature, the new hydrolysate free medium produces AAV that requires less purification, further decreasing the overall processing time and minimizing labor requirements. Furthermore, the TheraPEAK SfAAV Medium supports consistent cell growth throughout all phases of the culturing process, considerably reducing process variability.

As a chemically defined, non-animal origin product, the TheraPEAK SfAAV Medium is safer to use than media containing animal or human components, thereby facilitating regulatory compliance. Additionally, the medium is supplied with a U.S. Food and Drug Administration drug master file, alleviating the relevant preparation and submission burden.

Poster: Evaluation of AAV Production in Insect Cells Using Chemically Defined Media

A new poster developed by Lonza’s cell culture experts discusses the results of a study whereby the TheraPEAK SfAAV Medium was used to support the production of AAV in Sf9 insect cells. The study demonstrated the ability of the new medium to ensure lot-to-lot consistency, stable doubling time and increased AAV production.

The poster is free to download and can be accessed via the following link: https://bioscience.lonza.com/download/content/asset/36112

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