OGT pushes forward hybridisation-based targeted sequencing

OGT’s hybridisation expertise takes researchers from sample to sequencer in a single day

Oxford Gene Technology (OGT), The Molecular Genetics Company, has made several advances in hybridisation-based target enrichment protocols that enable researchers to prepare samples for sequencing in a single day.

Researchers can now access the high-quality results of hybridisation-based targeted sequencing with a speed comparable to that of amplicon-based approaches.

OGT’s hybridisation-based targeted sequencing delivers unparalleled coverage uniformity, enabling researchers to detect low frequency variants consistently down to 1% VAF (variant allele frequency) at a read depth of >1000x.

OGT’s hybridisation assay enhancements improve speed and also enable lower input levels of DNA to be used, allowing more researchers to precisely focus in on regions relevant to their research.

The company has optimised its enzymes and buffers to allow pooling of library preparation steps, enabling users to complete the whole enrichment protocol in less time.

This includes a short enzymatic fragmentation step, combined end-repair and adaptor ligation steps; and an optimised hybridisation of just 30 minutes for good quality DNA samples.

In addition, OGT’s NGS panel optimisation yields good data with as little as 10ng of starting material (subject to in-house validation).

Dave Cook, Senior Product Manager at OGT, said: “It’s clear that our hybridisation-based target enrichment has many advantages on amplicon methods in terms of quality and the ability to sequence through difficult regions, protocol length was a further area for improvement.”

OGT has also recently expanded its fully tested and optimised SureSeq myPanel NGS Custom Cancer Panel content, covering both haematology and solid tumours.

The new content provides optimised coverage for more than 90 genes, including difficult-to-sequence genes and other regions.