By denaturing the protein upon solvent addition, the protein crash technique used by the Porvair Sciences P3 microplate is highly effective at removing proteins from biological samples prior to analysis. The proteins “crash” or precipitate out of solution to form large, sticky globules that are then trapped on an open-pore structure filter.
Underneath this filter is a hydrophobic filter that retains any liquid above it. When a vacuum is applied to the P3 microplate, the liquid containing the analytes of interest is efficiently drawn through the filter into a collection plate — leaving behind unwanted proteins that can interfere with downstream applications.
The P3 microplate’s novel dual filter technology not only retains unwanted proteins and has good flow rates, it ensures that no leakage occurs prior to collection. Designed and precision manufactured to conform with ANSI /SLAS guidelines, the automation-friendly P3 microplate enables 96 samples to be filtered simultaneously, accelerating biological sample clean-up.
The P3 microplate is manufactured to ensure low extractable levels and prevent cross-contamination or loss of sample integrity.