EMBL develops automated pipeline of multi-protein complexes
Researchers at the European Molecular Biology Laboratory (EMBL) have developed a fully automated pipeline for the production of multi-protein complexes (ACEMBL) on Tecan’s Freedom EVO platform.
The Freedom EVO series offers four worktable capacities of 75, 100, 150 and 200cm, each with building-block modularity that brings precision, reliable liquid handling and easy-to-use robotics. Each platform can be combined with a choice of robotic arms, liquid handling tools and application options.
Tecan, a Swiss supplier of laboratory instruments, says the system enables users to perform experiments that were previously unthinkable using manual methods. It also has the potential to reveal unknown molecular mechanisms of health and disease that could be vital in drug discovery.
Dr Imre Berger, Group leader at EMBL, said: ‘Automation is the essential solution for protein complex expression and production, when manifold variation in the individual proteins is required for experimental success. With a robot, many experiments can be performed simultaneously and in parallel, with a precision that is unattainable manually. We use a Freedom EVO 200 platform with three identical liquid handling modules and, additionally, a sterile, covered compartment for growing cell cultures.’
Berger added that the two main advantages of automation are diversification and throughput.
‘We aim to produce a routine and robust high throughput application with several automated platforms running in parallel, repeating the ACEMBL procedure many thousands of times with up to 95% efficiency,’ he said.