Biotium has announced the launch of GlycoLiner Cell Surface Glycoprotein Labelling Kits for rapid, selective and covalent labelling of cell surface glycoproteins.
Unlike conventional aminooxy reagents, which are slow and require harsh buffers, catalysts or high label concentrations, GlycoLiner Kits use a gentler self-catalysing aminooxy chemistry for efficient labelling at neutral pH.
Surface labelling is completed in around 20 minutes at room temperature or 4°C, supporting workflows that require low temperatures to prevent protein internalisation.
The kits offer biotin or five bright fluorescent dye options and deliver covalent labelling compatible with standard fixation, permeabilisation and protein extraction protocols.
This enables a range of downstream applications, including immunofluorescence, microscopy, flow cytometry and biochemical analysis.
GlycoLiner also shows reduced intracellular staining in dead cells, providing clearer cell boundary visualisation than many other covalent cell surface stains.
"Biotium has launched an extensive selection of unique membrane-labelling probes that support different workflows."
"We are excited to provide yet another option that offers highly selective imaging of live cell surface glycoproteins that is also compatible with fixation,” said Dr Lori Roberts, Director of Bioscience at Biotium.
Key features include the following:
- Fast, simple and gentle: labels cell surface glycoproteins for imaging or protein analysis
- Highly selective live-cell surface labelling: lower dead-cell background than other covalent stains
- Compatible with fixation, permeabilisation and cell lysis: ideal for downstream imaging or analysis
- Novel self-catalysing reaction: efficient labelling at neutral pH without a catalyst
- Versatile labelling options: choose from five fluorescent dyes, from blue to near-IR, or biotin
- Efficient workflow: complete in ~20 minutes
GlycoLiner expands Biotium’s portfolio of innovative cell staining technologies, offering researchers a powerful new tool for cell surface biology research with clarity and precision.
GlycoLiner Kits are now available through Biotium and authorised distributors worldwide.