Porvair Filtration Group has launched a range of BioVyon products with immobilised Protein A for use in IgG antibody purification.
BioVyon Protein A microplates and columns offer a simple and rapid method for antibody purification, the company says. They also give a consistent recovery of IgG antibody that is independent of the flow rate during loading. Unlike existing bead-based technologies, there are no time-consuming slurries to equilibrate and pour into columns, which speeds up the protein purification process.
Each mini column has a capacity of approximately 2.5mg IgG (micro - 10µg IgG) and can be reused several times with appropriate washing steps before the performance degrades, the company adds. The BioVyon solid state media means that performance is not diminished at flow rates as high as 3ml/min, resulting in faster purifications.
For small-scale preparations, such as screening for IgG expression across a series of cell lines, in which sample is limited and high recoveries are needed, the consistency of BioVyon Protein A Mini and Micro Columns enables unmatched performance, the company adds.
Each well in the new product has a volume of approximately 1ml and can be used in a stand-alone mode with a vacuum manifold and collection plate, or as part of a fully automated system.
BioVyon is made from a pharmaceutical grade polyethylene material.
Porvair
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