The new 'chromatography flow-mode' option for Malvern's Zetasizer Nano particle and molecular characterisation system, enables its use as an in-line chromatography detector for protein applications.
'Chromatography flow-mode' permits the coupling of dynamic light scattering with size exclusion chromatography, allowing the operator to use Absolute Size Exclusion Chromatography (ASEC). Protein size can be measured directly, meaning that molecular weight standards are no longer required to identify oligomeric species or determine protein purity.
To use the Zetasizer Nano as an absolute light scattering detector requires connecting it after the last detector in a size exclusion chromatography (SEC) system. The instrument can be added to any SEC set-up; no column calibration is needed.
Size is plotted in real time as material is eluted from the column, and the molecular weight of each peak is calculated automatically at the end of the measurement.
By applying the system's patented optical design and high sensitivity capabilities to real time flow measurements, Malvern has achieved the Zetasizer Nano's use as a chromatography detector, providing a new tool for protein researchers.