Automation boosts sample preparation for next-generation sequencing

Published: 12-Feb-2010

Tecan has worked with Applied Biosystems, part of Life Technologies Corporation, to develop an off-the-shelf system for automated bead washing, based on Tecan's Freedom EVO 75 workstation equipped with a Te-MagS magnetic bead separation module.

Tecan has worked with Applied Biosystems, part of Life Technologies Corporation, to develop an off-the-shelf system for automated bead washing, based on Tecan's Freedom EVO 75 workstation equipped with a Te-MagS magnetic bead separation module. Automation of ePCR purification for the Applied Biosystems" SOLiD System next-generation genetic sequencing platform reduces the manual sample preparation.

Preparation of templated beads for the SOLiD System is a multi-step process, requiring several washes of magnetic beads for ePCR product purification. In a collaboration between Tecan and Life Technologies, a new system based on the Freedom EVO 75 workstation has been carefully developed to reduce manual variation and improve consistency.

The Freedom EVO 75 bead washing station is equipped with a two-channel LiHa arm, a Te­MagS magnetic separation module and all the racks and carriers required for parallel preparation of 24 templated bead samples. In addition, the system's Freedom EVOware software features washing protocols specifically developed by Life Technologies" Genetic Systems Division for optimal sequencing performance of the SOLiD System.

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