To recover large molecular weight proteins, such as blood factors and conjugated proteins or viruses and virus-like particles more effectively, Sartorius Stedim Biotech (SSB) has reconfigured its Sartobind membrane chromatography capsules. A range of capsule sizes with a 4mm bed height is now available from the 1mL nano unit up to the new 2.5L jumbo version.
The capsules’ upstream flow channels have been optimised and an internal core forms a miniaturised downstream channel, resembling that in existing 8mm bed height capsules. The new Q, S and salt-tolerant STIC PA ion exchanger capsules, all with a 4mm bed height, increase dynamic binding capacity by approximately 15% and reduce void volumes by around 40% compared with their predecessors, while maintaining high flow rates of 10 to 30 membrane volumes a minute.
By improving breakthrough behaviour, subsequent polishing steps to remove DNA, HCP, aggregates and viruses from recombinant proteins are significantly more reliable and, as less buffer is used, Sartobind also reduces operational costs, the company says.
'Traditionally, membrane adsorbers have been using available filter housings, often ignoring chromatographic process parameters, such as back-mixing effects and elution volumes. This new generation of membrane adsorber capsules takes these specific requirements into account and reflects substantial progress for bind and elute applications,' said Dr Fischer-Frühholz, membrane chromatography expert at SSB.
In addition, Sartorius Stedim Biotech has added new 400mL and 800mL Sartobind Q, S and Phenyl capsules with an 8mm bed height to its portfolio. These capsules increase dynamic binding capacity by up to 48% compared with adsorbers installed in filter housings.
