Rapid spin column from Sigma-Aldrich
Sigma-Aldrich has launched the Proteomics Grade Trypsin Spin Column, which the company says reduces the time taken to digest samples prior to mass spectrometry analysis.
Sigma-Aldrich has launched the Proteomics Grade Trypsin Spin Column, which the company says reduces the time taken to digest samples prior to mass spectrometry analysis.
The Trypsin Spin Column reduces the time to digest complex protein mixtures from as much as 18 hours with conventional methods to only 15 minutes. Potential discoveries can now be made in minutes rather than hours or days.
'With this new trypsin format, mass spectrometrists will now have the flexibility to analyse important samples within minutes,' said Jennifer Williams, product manager for quantitative proteomics at Sigma. 'The Trypsin Spin Column exemplifies our commitment to providing cutting-edge proteomics tools to the research community and to the development of innovative products in order to progress scientific research,' she added.
The new product contains highly purified, TPCK-treated porcine trypsin, immobilised on a chemically modified silica support to minimize non-specific absorption. This enables protein trypsination in one simple step; samples are ready for immediate analysis by mass spectrometry.
10-100 micrograms of protein sample are applied to the Spin Column, where they are digested with great efficiency and specificity. Fifteen minutes later, peptide fragments are eluted, ready for MS analysis and requiring no additional clean-up, Sigma says.
The company claims that the quality of MS data obtained using the Trypsin Spin Column is equal to, and in some cases better than, that obtained with a traditional digest.