Rohm & Haas goes peptide polishing

Rohm & Haas has introduced Amberchrom HPR10 - a polymeric media developed for large-scale polishing purification of therapeutic peptides and proteins.

Designed to be chemically and mechanically stable, highly selective and cost-effective, Amberchrom HPR10 is a 10µm monosized polymeric resin with a pore size optimised for the production of peptides, proteins and oligos.

To be used in large-scale HPLC equipment, polishing media must be able to withstand

pressures between 20 and 60 bar. The new technology is compatible with high-pressure requirements, offering much higher flowrate capacity, and therefore productivity, compared with traditional polymers.

Another critical factor is chemical stability. Amberchrom HPR10 is a highly crosslinked polymer, with a chemically inert surface that, unlike silica gels, can be cleaned and sanitised in place according to rigorous protocols with 1.0 N NaOH. This means that not only can the column be cleaned routinely for prolonged lifetime, but that manufacturing equipment can be fully cleaned and sanitised to prevent product carrryover, and endotoxin and viral contamination.

The lack of resolving power of earlier polymers prevented their use in polishing so Rohm & Haas created calibrated, monospheric 10mm beads with high capacity, selectivity and resolution power. The product is designed with selective pores for peptides and small proteins.