Fed up of time consuming and costly spin columns or extraction processes to prepare your sample for DNA amplification? Anachem now has the solution with new DNAreleasy.
This new cell lysing reagent requires just a 10 minute incubation on a thermal cycler before the DNA is ready for use directly in your PCR - not further sample processing is necessary.
DNAreleasy has been successfully used to lyse a wide range of cells. This includes:
human buffy coat, human fibroblasts tissue culture cells, human sperm, mouse & rat tissue culture cells, mouse tail, various plants, Drosophila and others like yeast, Gram +ve bacteria, Gram -ve bacteria, viruses and many more.
Using DNAreleasy is real easy. Just mix cells with the reagent and overlay with mineral oil if necessary (for Thermal Cyclers without a heated lid). Place in a Thermal Cycler and treat at 75°C for 5 minutes followed by 96°C for 2 minutes before holding at 20°C. After the lysis, a part or all of the DNAreleasy-lysate can be used directly in PCR. DNAreleasy can make up to 40% of most PCR mixes. It can be stored at -20°C for future use.
